Spectrophotometric determination of lipases, lysophospholipases, and phospholipases.

Recombinant Lipases and Phospholipases and Their Use as Biocatalysts for Industrial Applications

Lipases and phospholipases are interfacial enzymes that hydrolyze hydrophobic ester linkages of triacylglycerols and phospholipids, respectively. In addition to their role as esterases, these enzymes catalyze a plethora of other reactions; indeed, lipases also catalyze esterification, transesterification and interesterification reactions, and phospholipases also show acyltransferase, transacyla...

Evaluation of Expression of Lipases and Phospholipases of Malassezia restricta in Patients with Seborrheic Dermatitis

BACKGROUND Malassezia species (spp.) are cutaneous opportunistic pathogens and associated with various dermatological diseases including seborrheic dermatitis, dandruff and atopic dermatitis. Almost all Malassezia spp. are obligatorily lipid-dependent, which might be caused by lack of the myristic acid synthesis. Recent genome analysis of M. restricta and M. globosa suggested that the absence o...

Lipolytic Enzymes Involved in the Virulence of Human Pathogenic Fungi

Pathogenic microbes secrete various enzymes with lipolytic activities to facilitate their survival within the host. Lipolytic enzymes include extracellular lipases and phospholipases, and several lines of evidence have suggested that these enzymes contribute to the virulence of pathogenic fungi. Candida albicans and Cryptococcus neoformans are the most commonly isolated human fungal pathogens, ...

Lipases and Phospholipases in Drug Development From Biochemistry to Molecular Pharmacology

Extraction of Traces of Copper as Tetra-Butylammonium Tetrathiocyanato Copper (II) and Its Spectrophotometric Determination Using Eriochrome Black T

An extraction-spectrophotometric method for the determination of traces of copper (II) is described. The method is based on the quantitative extraction of copper as tetrabutylammonium tetrathiocyanato copper (II) into methyl isobutyl ketone, followed by spectrophotometric determination, using eriochrome black T as a metallochromic indicator. The red-violet mixed complex of copper absorbs at...